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. 2019 Feb 25;10:287. doi: 10.3389/fimmu.2019.00287

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Copyright © 2019 Guo, Liu, Yin, Kan, Gong, Li, Cao, Wang, Xu, Ma, Fu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of licochalcone A on AKT/NF-κB signaling pathway in LPS-induced mice mastitis. Mammary gland tissues from different experimental groups were obtained 24 h after LPS administration and total protein. The tissue lysates were prepared and subjected to western blot by using p-AKT (A,B), p-IκBα (A,C), and p-P65 (A,D) antibodies, respectively. Each immunoreactive band was digitized and expressed as a ratio of the β-tubulin level. Values are presented as means ± SD (*p < 0.05, ***p < 0.001, and ****p < 0.0001 vs. LPS group).