Figure 7.

Depletion of lymphocyte antigen 6 complex locus G6D (Ly6G)‐expressing neutrophils does not ameliorate the haemophagocytic lymphohistiocytosis (HLH)‐like syndrome in mouse cytomegalovirus (MCMV)‐infected wild‐type (WT) mice. Anti‐Ly6G‐antibodies (clone 1A8) or immunoglobulin (Ig)G2a isotype control (clone 2A3) were injected intraperitoneally on day −1 prior to infection. (a) Absolute neutrophil count in whole blood in the HLH model, in WT and interferon (IFN)‐γ knock‐out (KO) mice, 5 days post‐infection (p.i.). (b) Absolute neutrophil count in whole blood of WT mice, after anti‐Ly6G‐mediated depletion, day 5 post‐infection (p.i.) (c) Absolute count of Gr1‐expressing neutrophils in lung, gated as Gr1⁺ cells of live (ZombieAqua^–) singlet lung cells, day 5 p.i. (d) Percentage change in body weight relative from initial body weight at day 0. Median with interquartile range of five mice per group. (e) Rectal body temperature (temp.) (°C) at day 2 p.i. Dotted line = 38·5°C. (f) Absolute lymphocyte, platelet and red blood cell (RBC) count in whole blood, day 5 p.i. (g) Plasma concentration of the ferritin heavy chain (ng/ml), day 5 p.i. Dots represent the average of two dilutions for one single mouse. (h) Plasma concentration of alanine transaminase (ALT) (IU/L), day 5 p.i. (a–c,e,f,h) Dots represent individual animals. Horizontal bars refer to medians. aLy6G = depleting monoclonal antibody; IgG2a = isotype control; NI = not infected; PBS = no antibody, only saline treatment; ns = not significant (P ≥ 0·05); *P < 0·05; **P < 0·01; Kruskal–Wallis with Dunn's post‐test. Depicted data are from one experiment.