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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Clin Cancer Res. 2018 Dec 17;25(5):1574–1587. doi: 10.1158/1078-0432.CCR-18-2036

Figure 1. Downstream mTORC1/mTORC2 substrates are differentially regulated in primary CLL cells isolated from distinct prognostic subgroups.

Figure 1

Protein lysates were generated from freshly-isolated B lineage cells (CLL/mature B cells) purified from peripheral blood of CLL patients or healthy donors. CLL patients were stratified based on cytogenetic abnormalities to indicate prognosis: normal/del(13q) (good), del(11q), del(17p) (poor). A. Western blotting was performed to determine the phosphorylated levels of mTORC1 downstream targets (S6S235/236, 4EBP1T37/46 and RICTORT1135) and mTORC2 substrate (AKTS473) together with GAPDH as a loading control. Representative n=2 for each subset shown. B. Densitometry ratios of phosphorylated proteins vs. GAPDH from the blot are shown. n≥6 for each patient subset, n≥4 healthy controls.