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. Author manuscript; available in PMC: 2019 Jun 28.
Published in final edited form as: Cell. 2018 May 31;174(1):72–87.e32. doi: 10.1016/j.cell.2018.05.009

Figure 2 -. Acidification is both necessary and sufficient to disrupt the clock network.

Figure 2 -

A. Rhythmicity, amplitude, period, and phase as a function of media pH determined by singlecell luminescence imaging of U2OS Arntl::dLUC cells. Mean ± SEM of ≥ 25 analyzed cells (13–25 rhythmic) each pH. One-way ANOVA/post-hoc Dunnett’s *p<0.05, ***p<0.001, ns=p>0.05. B-D. Expression (qPCR) of endogenous core clock components in synchronized U2OS Arntl::dLUC cells treated with 1 mM DMOG or vehicle in low (B) or high (C) buffer media or with pH 7.4 or 6.3 media (D). See also Figure S2.