Table 1.

Enzyme kinetic parameters for the formation of NA-GSH from NA by lung and liver microsomes from WT and LCN mice

Apparent Km and Vmax values for the microsomal formation of NA-GSH were determined as described in Methods. The results shown represent means ± S.D. of values determined for three separate microsomal samples, each prepared from tissues pooled from three 2-month-old male mice.

Tissue, strain	Km, μM	Vmax, nmol/min/mg of protein	Vmax/Km, ml/mg of protein/min
Liver
WT	4.71±0.76	1.10±0.04	0.23
LCN	5.09±0.89	0.12±0.01*	0.02
Lung
WT	13.6±1.6	1.35±0.05	0.10
LCN	8.96±1.17	1.27±0.04	0.14

^*p<0.01 (Student’s t-test) compared to WT mice