Skip to main content
. 2019 Feb 26;8:e44341. doi: 10.7554/eLife.44341

Figure 3. Identification of AMPs involved in the susceptibility of ΔAMPs flies to C. albicans.

Figure 3.

(A) Survival of mutants for groups of AMPs reveals that loss of only Toll-responsive Group C peptides (Metchnikowin and Drosomycin) is required to recapitulate the susceptibility of ΔAMPs flies. Co-occurring loss of groups A and C has a net protective effect (A*C: HR = −1.71, p=0.002). (B) Further dissection of Group C mutations reveals that both Metchnikowin and Drosomycin contribute to resist C. albicans survival (p=0.008 and p<0.001, respectively). The interaction of Metchnikowin and Drosomycin was not different from the sum of their individual effects (Mtk*Drs: HR = −0.80, p=0.116). (C) Fungal loads of individual flies at 18 hpi. At this time point, BomΔ55C mutants and spzrm7 flies have already failed to constrain C. albicans growth (C’). Fungal titres at 36hpi (C’’), a time point closer to mortality for many AMP mutants, show that some AMP mutants fail to control fungal load, while wild-type flies consistently controlled fungal titre. One-way ANOVA: not significant = ns, p<0.05 = *, p<0.01 = **, and p<0.001 = *** relative to iso w1118.