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. 2019 Jan 2;42(2):113–122. doi: 10.14348/molcells.2018.0430

Fig. 1. The expression of primary cilia is decreased in anaplastic thyroid carcinomas (ATCs).

Fig. 1

(A) Immunofluorescence (IF) analysis of primary cilia using ARL13B (green) for axonemes and GT335 (red) for axonemes with basal bodies. (B) The frequency of primary cilia was 32.60 ± 10.20% in Nthy-ori 3-1, 23.13 ± 3.74% in TPC1, 25.13 ± 4.25% in BCPAP, 12.28 ± 5.63% in 8505C, 12.46 ± 2.38% in SW1736, and 12.52 ± 3.23% in HTh7 cells, respectively. (C, D, E) IF analysis of primary cilia using ARL13B (green) for axonemes and γ-tubulin (red) for basal bodies. The frequency of primary cilia was 67.53 ± 3.62% in normal thyroid follicles, 68.74 ± 18.01% in PTC, and 1.87 ± 1.51% in ATC (normal vs. PTC, P = 0.2956; normal vs. ATC, P < 0.001). **P < 0.01; ***P < 0.001; NS, not significant. Scale bar: H&E, 100 μm; IF, 5 μm. (F) Activation of primary cilia-specific genes in the normal thyroid and thyroid cancers. Boxplots from the single sample gene set enrichment analysis (ssGSEA) show the activation levels of primary cilia-specific genes in the normal thyroid and in three subtypes of thyroid cancer. Abbreviations: FA/FTC, follicular adenoma/follicular thyroid carcinoma; PTC, papillary thyroid carcinoma; ATC, anaplastic thyroid carcinoma.