Fig. 1. Identification of a novel transcript of Lpar1 (transcript variant 3) and its transcription start site in neocortical neuroblast cells.

(A) Total RNA isolated from TR mouse cells (Chun and Jaenisch, 1996) was subjected to a 5′RACE reaction using 5′ universal primer mix and 3′ Lpar1-specific primers. Lane 1 represents a 1kb plus ladder and lane 2 represents the transcript variant 3 identified from 5′RACE analysis. (B) A schematic representation of transcript variant 3 with indicated positions of the alternatively spliced sequences of exon 1a, 1b and 1c. The asterisk shows the relative position of translation start site for LPA₁. (C) Transcript variant of Lpar1 gene expression was analyzed by quantitative reverse transcriptase polymerase chain reaction. Each cDNA from embryo and adult C57BL/6 mice tissue or TR cells was amplified using specific primers (n=3, versus transcript variant 1 in TR cells). Error bars indicate S.E.