Fig. 2. Putative cis-acting elements and reporter activity of the mouse Lpar1 promoter.

(A) These putative transcriptional regulation elements were identified from the Transcription Element Search System (TESS http://www.cbil.upenn.edu/tess/). (B) TR mouse neocortical neuroblast cells (Chun and Jaenisch, 1996) were transiently transfected with reporter plasmids, pGL3b (pGL3-basic) or (−2867/+225)-pGL3b. The cells were lysed and assayed for luciferase activity twenty-four hours post-transfection. The values represent the mean ± S.E. of increase in luciferase activity in folds with respect to the pGL3-basic construct. The experiment was performed in triplicate and values were normalized to the activity of Renilla luciferase, which was transfected concurrently (40 ng) in all the assays to measure transfection efficiency. **P < 0.01.