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. 2019 Jan 2;42(2):123–134. doi: 10.14348/molcells.2018.0399

Fig. 5. Involvement of HEB in negative regulation of the mouse Lpar1 promoter through E-boxes.

Fig. 5

(A) TR cells were co-transfected with luciferase constructs (−761/+225wt, Eb1mut, Eb2mut, Eb3mut, and Eb1/3mut) and the HEB expression vector. (B) TR cells were co-transfected with −761/+225 wt and expression vectors (HEB, Id3). The values represent the mean ± S.E. of the percentage of luciferase activity in the presence of HEB or Id3 expression vector relative to the luciferase activity in the presence of an empty vector. The experiment was performed in triplicate and the values were normalized to the activity of concurrently transfected Renilla luciferase. (C) After forty-eight hours, cells were transfected with siRNAs for no-silencing (Ctrl) and HEB. Cell lysates were prepared by western blot analysis for detection of HEB and β-actin. (D) TR cells were co-transfected with luciferase constructs (−761/+225wt and −350/+225wt) and siRNAs for no-silencing (Ctrl) or HEB. *P < 0.05, **P < 0.01, ***P < 0.001. ns is non-significant.