Table 1.
| Primers and the restriction enzyme used for generation of promoter reporter constructs. | ||
|---|---|---|
|
| ||
| Constructs | Primers | Restriction enzyme |
| −937/+225 | Forward: ACTATCCAACCCCCAGTGTCTT Reverse: TCTTGCAGAGCTTTGGTGGA |
SphI |
| −248/+225 | Forward: CTTGTTTTCCCCAGGCTCTGT Reverse: GCCGGGCCTTTCTTTATGTT |
XhoI |
| −660/+225 | Forward: GTGTGAAGCAGGTGGGTGAA Reverse: GCCACATCCACAGAGCACAA |
PstI |
| −539/+225 | Forward: CCGCCTACACTTCCAGGTG Reverse: GCCACATCCACAGAGCACAA |
PstI |
| −432/+225 | Forward: AGCTCTAGGGACACAAAGGC Reverse: GCCACATCCACAGAGCACAA |
PstI |
| −350/+225 | Forward: GCTGTGCTGGTTGAAACTTTGT Reverse: GCCACATCCACAGAGCACAA |
PstI |
|
| ||
| Restriction enzyme used for generation of promoter reporter constructs. | ||
|
| ||
| Constructs | Restriction enzyme | |
|
| ||
| −2867/+225* | SacI/KpnI | |
| −1766/+225* | NheI/KpnI | |
| −761/+225 | SauI/NheI | |
| −142/+225 | StuI/NheI | |
| −3/+225 | PstI/NheI | |
*
Constructs made from restriction enzyme digest of the −3549/+518 construct
All the other constructs were created by PCR and/or restriction enzyme digest of the −1766/+225 construct