Table 4.
Efflux pump genes and oprD detection and expression in clinical (n = 33) and non-clinical (n = 30) Pseudomonas aeruginosa strains isolated in this study.
| mexB | mexF | mexY | oprD | |
|---|---|---|---|---|
| Positive RT-qPCR detection | ||||
| Overall | 54 (86)a | 52 (83) | 57 (90) | 29 (46) |
| Clinical | 33 (100)∗ | 32 (97)∗∗ | 33 (100)∗∗ | 15 (45) |
| Non-clinical | 21 (70)∗ | 20 (67)∗∗ | 24 (80)∗∗ | 14 (47) |
| Overexpression | ||||
| Overall | 9 (14) | 4 (6) | 15 (24) | 0 (0) |
| Clinical | 9 (27)† | 4 (12) | 15 (45)∗ | 0 (0) |
| Non-clinical | 0 (0)† | 0 (0) | 0 (0)∗ | 0 (0) |
| Underexpression | ||||
| Overall | 39 (62) | 46 (73) | 35 (56) | 49 (78) |
| Clinical | 11 (33)∗ | 18 (55)† | 5 (15)∗ | 23 (70) |
| Non-clinical | 28 (93)∗ | 28 (93)† | 30 (100)∗ | 26 (87) |
RT-qPCR detection was considered positive for a given gene when cycle thresholds and subsequent Normalized Calibrated Ratios could be calculated from the experiments.
aNumber of strains (%).
∗Significant difference between clinical and non-clinical strains (p < 0.0001, Fisher’s exact test).
∗∗Significant difference between clinical and non-clinical strains (p < 0.001, Fisher’s exact test).
†Significant difference between clinical and non-clinical strains (p < 0.01, Fisher’s exact test).