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. 2019 Feb 26;10:128. doi: 10.3389/fphar.2019.00128

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Copyright © 2019 Deng, Guo, Wei, Gao, Zhou and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PNU-282987 treatment activation of α7nAChR mRNA and protein in lung macrophages. (A) Fluorescence quantitative real-time polymerase chain reaction (RT-PCR) showing the relative level of α7nAChR mRNA in lung macrophages treated with or without LPS+ATP, and PNU-282987. (B) Representative western blot showing the expression of α7nAChR (56 kDa) protein in lung macrophages treated with or without LPS+ATP, and PNU-282987. (C) Relative protein levels of α7nAChR were determined after normalization to GAPDH. LPS, lipopolysaccharide; ATP, adenosine-triphosphate. Data were accumulated from three independent assays. The data are expressed as means ± SD. ^∗P < 0.05 versus the control cells and #P < 0.05 versus LPS+ATP cells.