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. 2019 Feb 26;10:133. doi: 10.3389/fphar.2019.00133

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Copyright © 2019 Boudieu, Mountadem, Lashermes, Meleine, Ulmann, Rassendren, Aissouni, Sion, Carvalho and Ardid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of pregabalin (PGB; 30 mg kg^-1, s.c.) on cystitis-induced mechanical referred hyperalgesia assessed by the abdominal von Frey test. The von Frey filaments were applied to the lower abdominal area close to the urinary bladder. Median 50% threshold (T50) was determined by the up-and-down method. The values are expressed as a mean ±S.E.M. and compared by a 1-way ANOVA (Treatment) followed by Tukey post hoc test for multiple comparisons. N = 8/group. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control group.