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Subcellular localization and transactivation activity of SlDOF10 protein. (A,B) Nuclear localization of the YFP-SlDOF10 and SlDOF10-YFP fusion proteins. (C) Transient dual-LUC reporter assays showing SlDOF10 ability to activate transcription. A reporter construct with or without DOF binding sites (2 × DOF motif) was coexpressed with SlDOF10 protein and or the corresponding empty vector in the indicated combinations. 35S:LUC was used as a positive control.
