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. 2019 Mar 4;9:3357. doi: 10.1038/s41598-019-39769-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of LIMKs in WT mouse cochlea. (a) Immunofluorescence staining showed that LIMK1 and LIMK2 were expressed in the cochlear epithelium in the P21 and P30 mice. Myosin7a was used as a marker for HCs. Images were taken from the basal turn of the sensory epithelium. There was no difference in the immunolabeling of LIMK1 and LIMK2 from the apical to basal turns. Scale bar = 10 µm. (b) RT-qPCR results show the changes in expression of Limks in the mouse cochlea from embryonic development to adult. β-actin was used as the internal control. Data are presented as mean ± SD. (p < 0.01, n = 5).