Figure 2.

Analysis of LIMK expression in the DKO mice at the mRNA and protein level. (a) The cochleae of P3 DKO mice were immunolabeled with LIMK1 and LIMK2 antibodies. Scale bar = 10 µm. Images were taken from the basal turn of the sensory epithelium. There was no difference in the immunolabeling of LIMK1 and LIMK2 from the apical to basal turns. (b) RT-PCR was performed to analyze the DKO mice. Total cochlear RNA was extracted from P3 DKO and WT mice. Brn3.1 was used as the positive control, and β-actin was used as the internal control. (c) Western blot was performed to analyze DKO mice using antibodies against LIMK1 and LIMK2. Proteins from the brain and cochlea were extracted from P3 DKO and WT mice, and GAPDH was used as the internal control.