Figure 6.

The effect of loss of LIMKs on cofilin. (a) Western blot was performed to detect the expression level of cofilin and p-cofilin in both P30 WT and DKO mice, n = 3. (b) Quantification of the Western blot (p < 0.01). (c) Auditory HCs of P30 DKO and WT mice were stained with cofilin and p-cofilin antibodies. Images were taken from the basal turn of the cochlea, and there was no difference from the apical to basal turns. Scale bar = 10 µm. (d) Quantification of cofilin and p-cofilin immunolabeling in the basal turns of OHCs (p < 0.05) n = 3. (e) RT-qPCR was performed with P30 WT and DKO mice cochleae (p < 0.05, n = 5). Data are presented as mean ± SD.