Table 3.
Modelled and adopted optimum conditions for the significantly influential experimental factors, partial and global desirability of the peak areas, the predicted areas (and their 95% confidence limits) and the experimentally obtained peak areas.
| Factor | Setting | Modified | |||||
|---|---|---|---|---|---|---|---|
| Desorption flow (A), mL/min | 95.1069 | 95 | |||||
| Cryofocusing Temperature (B), °C | 31.71 | 20 | |||||
| Cryo-desorption Temperature (C), °C | 294.698 | 295 | |||||
| Response | Optimized | Prediction | Lower 95.0% Limit | Upper 95.0% Limit | Desirability | Validation (±SD, n = 3) | CV (%) of validated experimental values |
| TEP | yes | 1061000.0 | 648994.0 | 1474000.0 | 0.4786 | 978917.0 ± 112894.0 | 11.5 |
| TPP | yes | 4620000.0 | 3513000.0 | 5728000.0 | 0.8734 | 3702588.0 ± 334614.0 | 9.0 |
| TNBP | yes | 3243000.0 | 2480000.0 | 4007000.0 | 0.7477 | 3046976.0 ± 301440.0 | 9.9 |
| TCEP | yes | 739188.0 | 621161.0 | 857215.0 | 0.7102 | 595104.0 ± 49439.9 | 8.3 |
| TCIPP | yes | 362965.0 | 276627.0 | 449303.0 | 0.6574 | 326112.0 ± 21869.2 | 6.7 |
| TDCIPP | yes | 301971.0 | 252442.0 | 351500.0 | 0.5049 | 241696.0 ± 18558.9 | 7.7 |
| TBOEP | yes | 285892.0 | 243703.0 | 328081.0 | 0.5896 | 217899.0 ± 26699.6 | 12.3 |
| TPHP | yes | 1275000,0 | 1026000.0 | 1524000.0 | 0.8499 | 1107435.0 ± 90919.8 | 8.2 |
| EHDPP | yes | 3015000.0 | 2617000.0 | 3413000.0 | 0.8568 | 2295061.0 ± 260412.0 | 11.3 |
| TEHP | yes | 8525000.0 | 7159000.0 | 9890000.0 | 0.9383 | 6339173.0 ± 682202.0 | 10.8 |
| Global desirability | 0.7299 | ||||||