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. 2019 Mar 4;10(3):215. doi: 10.1038/s41419-019-1471-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The liver tissues from the wild-type (WT) mice harvested at ZT 5 and ZT 13 were labeled with anti-PABP1 (stress granule marker) antibody and revealed by immunofluorescence. Mice were intraperitoneally injected with saline or sodium arsenite (SA, 10 mg/kg) for 1 h before killing. Representative micrographs showing the presence of PABP1-positive stress granules (Scale bar = 10 μm). b, c Quantification of all visible PABP1 puncta (b) or large size puncta (>0.5 μm, c) respectively (n = 3 mice per group with 950–1000 cells examined per mouse. mean ± S.E.M.; two-way analysis of variance (ANOVA) with Tukey’s multiple comparison *P ≤ 0.05, ***P ≤ 0.001)