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. 2019 Mar 4;10(3):215. doi: 10.1038/s41419-019-1471-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of the strategy of tagging green fluorescent protein (GFP) at the N terminus of human G3BP1 using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9). b Immunofluorescence confocal microscopy showing the co-localization of endogenous SG marker PABP1 with knock-in GFP-G3BP1. Stress granules were induced with 100 μM sodium arsenite for 1 h. The square areas in the middle panels were enlarged and shown in the right. Scale bar = 20 μm