Fig. 3. SM promote a mesenchymal transition in GSCs.

a Relative mRNA expression of CD44 in 157 GSCs treated with BIR (10 µM) at 3 and 8 days, as determined by qRT-PCR. b MGG23 GSCs were treated with BIR (10 µM) for 7 days, then analyzed for CD44 and CD133 expression by flow cytometry. The bar graph depicts the percentage of CD44 high cells in Ctrl and BIR-treated GSCs. A representative result from three independent experiment is shown. c Relative CD44 mRNA expression in 157 GSCs expressing shSCR or shTNFα and treated with BIR (10 µM) for 7 days. d Relative mRNA expression of ALDH1A3, Vimentin, and MMP9 in 157 and 19 GSCs treated with BIR (20 µM) for 4 days. e Cell viability of MGG8 GSCs treated with BIR in the presence or absence of DEAB at the indicated doses for 3 days. *p < 0.05; **p < 0.001; Student t-test