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. 2019 Mar 4;5:72. doi: 10.1038/s41420-019-0155-9

Fig. 4. Activation of STAT3 signaling following treatment with SM.

Fig. 4

a Western blot analysis of pY-STAT3 and STAT3 in MGG23 treated with BIR overtime. b Western blot analysis of pY-STAT3 and STAT3 in four GSCs treated with BIR or TNFα for 48 h. c mRNA expression of STAT3 and NF-κB transcriptional targets in MGG8 and BT07 GSCs treated with BIR for 48 h. Data are normalized to HPRT and expressed as fold change compared to the untreated control. d The expression of a constitutively active STAT3-C in GSCs leads to increased pY-STAT3 and STAT3 levels as determined by western blot. e Relative mRNA expression of the indicated STAT3 and NF-κB transcriptional targets in MGG8 GSCs expressing a control lentivirus (Ctrl) or STAT3-C. f Western blot analysis of pY-STAT3 and STAT3 in 83 GSCs expressing shSCR, shTNFα or shIL-6 and treated with BIR for 48 h