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. 2019 Mar 4;10(3):224. doi: 10.1038/s41419-019-1456-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The affinities of the indicated TNFR2-specific mIgG1 antibodies were evaluated by binding studies with TNFR2-Fc-GpL and plastic immobilized antibodies. b Co-cultures of HeLa-TNFR2 cells and murine CD32B-expressing HEK293 cells were challenged overnight with the indicated concentrations of the TNFR2-specific mIgG1 antibodies. Finally, IL8 production was quantified by an ELISA