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. 2019 Mar 4;10(3):224. doi: 10.1038/s41419-019-1456-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Domain architecture and structural organization of C4-IgG1(N297A)-HC:scGITRL, C4-IgG1(N297A)-HC:sc(mu)4-1BBL, C4-IgG1(N297A)-HC:(mu)GITRL, and C4-IgG1(N297A)-HC:muIL2. b HeLa-TNFR2 cells and HEK293 transfectants expressing the indicated cytokine receptors were stimulated with 200 ng/ml of the various C4-IgG1(N297A) cytokine fusion proteins in the presence and absence of 1 µg/ml protein G. Next day, IL8 content of supernatants were determined by ELISA. c Co-cultures of HeLa-TNFR2 cells and the various HEK293 transfectants were stimulated as indicated with the C4-IgG1(N297A) cytokine fusion proteins and IL8 production was again determined the following day by ELISA