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. 2019 Mar 4;9:3360. doi: 10.1038/s41598-019-40214-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time course of AM-responsive gene expression. 28 days after germination, plants were mycorrhized and harvested at the time points indicated. Plants at 0 days post inoculation were harvested after 3 h of inoculation with R. irregularis spores. At each time point, three biological replicates were harvested, each consisting of 6 pooled root systems. Relative gene expression levels were determined by real-time RT-PCR. The standard error of the mean is indicated.