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. 2019 Mar 4;9:3359. doi: 10.1038/s41598-019-40044-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Two approaches to generate M18-L-ST·SC-L-DBD for affinity matrix preparation. Generation of the covalently linked M18-L-ST·SC-L-DBD complexes by mixing (a) M18-L-ST with excess SC-L-DBD or (b) SC-L-DBD with excess M18-L-ST. M18, SAVSBPM18; ST, SnoopTag; SC, SnoopCatcher; L, Linker; DBD, Dextran-binding domain.