Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 4;9:3359. doi: 10.1038/s41598-019-40044-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Purification of SBP-tagged β-lactamase using M18-L-DBD affinity matrix under a non-overloading condition. (a) Analysis of different fractions from the column by SDS-PAGE. S, B. subtilis culture supernatant containing overproduced SBP-tagged β-lactamase; FT, flow-through fraction; W, wash fractions; E, elution fractions. Open arrowhead indicates SBP-tagged β-lactamase. (b) SDS-PAGE of the bound fraction from the M18-L-DBD Sephadex column used for purification of SBP-tagged β-lactamase. Lane 1 is the boiled matrix before sample loading; lane 2 represents the boiled matrix after sample loading and before elution with biotin; lane 3 is the boiled matrix after elution with biotin. Closed arrowhead, M18-L-DBD; Open arrowhead, retained SBP-tagged β-lactamase. M, molecular weight markers (sizes in kDa). Gel profiles shown in panels a and b are from different gels.