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. 2019 Mar 4;9:3331. doi: 10.1038/s41598-019-39219-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P. a.-inhibitory antimicrobials in stratum corneum. (a) reversed-phase-HPLC of heparin-bound proteins of an acidic stratum corneum extract. (b) radial-diffusion antimicrobial assay (RDA) of HPLC fractions for P. a. ATCC 10145 inhibitory activity at pH 7.4. (c) RDA of HPLC fractions for P. a. inhibitory activity at pH 5.5 and test for immunoreactive HRNR. P. a.-inhibitory activity at pH 7.4, corresponding to known skin antimicrobial peptides like RNase-7, an hBD-3 variant, hBD-2 and psoriasin is shown in (b). Results are representatives of three (b,c) independent experiments.