Fig. 1.

The agglomerates were formed by Trizol method but not with MTM method. a Trizol method: add 1 ml TRIzol and 200 µl chloroform into the grinding tuber, centrifuge at 12,000 rpm, 4 °C for 15 min after incubate for 3 min at room temperature; b MTM methods: grinding tuber is incubated with RNA extraction buffer, β-mercaptoethanol, and absolute ethanol, chloroform. After centrifuge, add 1 ml Trizol and 200 µl chloroform into supernatant, and centrifuge at 12,000 rpm, 4 °C for 15 min