Table 1.

Primers used for detection of cry1A, cry1B, cry2A, cry9, vip3A, and chi36 genes

Target gene(s) recognized	Primer name	Sequence 5′ → 3′	Product size (bp)	T a (°C)	References
cry1Aa, cry1Ab, cry1Ac cry1Ad, cry1Ae, cry1Af, cry1Ag	Cry1A-f	ATTCGCTAGGAACCAAGC	398	55	Thammasittirong and Attathom (2008)
	Cry1A-r	AATCCGGTCCCCATACAC
cry1Ba, cry1Bb, cry1Bc, cry1Bd, cry1Be, cry1Bf	Cry1B-f	CAGAAACAACAGAACGACC	921	57	Thammasittirong and Attathom (2008)
	Cry1B-r	CACTTCCCCACCATCCAT
cry2Aa, cry2Ab, cry2Ac, cry2Ad	Cry2A-f	TACCTTTATTTGCACAGGCA	1170	54	Thammasittirong and Attathom (2008)
	Cry2A-r	CTACCGTTTATAGTAACTCG
cry9A, cry9B, cry9C, cry9D, cry9E	Cry9-f	CACATGAGTTTTCTTCCTAT	440	54	Thammasittirong and Attathom (2008)
	Cry9-r	AGATACGATGCTTGTTGTAA
vip3A	Vip3A-f	GGATTTGCCACTGGTATCAAAG	1591	53	The current work
	Vip3A-r	TTGCTTTCCACGGCTCTA
chi36	Chi36-f	GATGTTAAACAGGTTCAA	1083	50	Arora et al. (2003)
	Chi36-r	TTATTTTTGCAAGGAAAG