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. 2019 Feb 26;13:171. doi: 10.3389/fnins.2019.00171

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Copyright © 2019 Alonso, Pisa and Carrasco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry to detect C. pneumoniae antigens in brain tissue from ALS patients. Double immunostaining and confocal microscopy were carried out as indicated in section Materials and Methods. CNS sections were immunostained with a rabbit polyclonal C. pneumoniae antibody (green) (1:20 dilution) and a rat polyclonal anti-T. viride antibody (red) (1:20 dilution). DAPI staining of nuclei appears in blue. Scale bar: 5 μm. (A,B) ALS2; (C,D) ALS3; (E) ALS5; (F–H) ALS6; (I,J) ALS1; (K) ALS7; (L,M) ALS8; (N) ALS9; (O) ALS10; and (P) ALS11. (A,C,F,I,K,L,P) MC section; (G,N,O) MD section; and (B,D,E,H,J,M) SC section.