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. 2019 Feb 26;10:233. doi: 10.3389/fpls.2019.00233

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Copyright © 2019 Hung, Chen, Li, Chen, Chen, Cui and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LDL1/LDL2 interact with TOC1. (A) BiFC assays in Arabidopsis protpplasts showing interaction between LDL1/LDL2 and TOC1 in living cells. LDL1, LDL2, and TOC1 fused with the N terminus (YN) or C terminus (YC) of YFP were co-delivered into Arabidopsis protpplasts. The nucleus was indicated by mCherry carrying a nuclear localization signal. (B,C) Yeast two hybrid analysis of the interaction of LDL1/LDL2 with TOC1. LDL1-BD/LDL2-BD with CCA1-AD or LHY-AD was co-transformed into the yeast strain AH109. The transformants were plated on the SD/-Leu-Trp-His medium. (C) Quantitative α-galactosidase assays for protein-protein interaction in yeast. Bars indicate SD from three biological replicates. (D) Co-IP of the native promoter driven LDL1:GFP with TOC1 in LDL1pro::LDL1:GFP transformed Arabidopsis protoplasts. Western blot (WB) was performed with the anti-FLAG and anti-GFP antibodies.