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. 2019 Feb 26;10:233. doi: 10.3389/fpls.2019.00233

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Copyright © 2019 Hung, Chen, Li, Chen, Chen, Cui and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LDL1/2-HDA6 is involved in regulation of CCA1/LHY. (A) Expression of CCA1 and LHY in TOC1-OE plants, hda6/ldl1/2, and WT. Gene expression levels were determined by qRT-PCR and normalized to UBQ10. Plants were grown under 12/12 light/dark for 14 days and collected on ZT0. (B) Transient luciferase assays in CCA1pro::CCA1:LUC (pCCA1:LUC) transformed protoplasts. CaMV 35S promoter driven TOC1, HDA6, or LDL1 effector constructs were introduced into mesophyll protoplasts. Samples were collected on ZT0 after 12 h of transformation. Relative Light Units (RLU) represents firefly luciferase normalized by co-expressed 35S pro::Renilla luciferase. 35Spro::GFP transformed protoplasts were used as the negative control. Data points represent the average of three technical replicates. Error bars correspond to SD from three biological replicates. ^∗P < 0.05, ^∗∗P < 0.005 (Student’s t-test).