Figure 5.

Effects of ISE on LPS-induced MAPK and NF-κB signals. RAW264.7 macrophages were pretreated with different concentrations of ISE for 1 h and then stimulated by 1.0 μg/mL LPS for 30 min. Total cell lysates were extracted, and then western blotting using specific antibodies was used for the detection of phosphorylated and total forms of three MAPK molecules, ERK (A), JNK (B), p38 (C), and I-κB-α (D). The value of a control was set at 100%, and the relative value was presented as fold induction to that of the control, which was normalized to total MAPK or β-actin. The values are means ± SD, n = 3. Statistical comparisons were made with each vehicle. ^*P < 0.01, ^**P < 0.001.