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. 2019 Feb 26;10:286. doi: 10.3389/fimmu.2019.00286

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Copyright © 2019 Zhang, Xie, Su, Liu, Zhang, Pang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of ISE on 3T3L1 CM-induced MAP kinase and NF-κB signals. RAW264.7 macrophages were pretreated with different concentrations of ISE for 1 h and then stimulated by 3T3-L1 CM (L1CM) for 30 min. Total cell lysates were extracted to detect the levels of phosphorylated ERK (A), JNK (B), p38 (C), and I-κB-α (D). The value of a control was set at 100%, and the relative value was presented as fold induction to that of the control, which was normalized to total ERK, JNK, p38 or β-actin. The values are means ± SD, n = 3. Statistical comparisons were made with each vehicle controls. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.