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. 2019 Feb 26;10:299. doi: 10.3389/fimmu.2019.00299

Figure 2.

Figure 2

Shorter TCRβ CDR3s with fewer insertions were enriched during thymic selection. (A–D) Comparison of productive and out-of-frame TCRβ rearrangements revealed higher frequencies of short TCRβ CDR3s (and lower frequencies of long ones) in the Post-selection repertoires of 4RA (A), 4RO (B), 8RA (C), and 8RO (D). Mixed effects two-way ANOVA with individual as random variable. (E) The percentage of short TCRβ CDR3s clonotypes (16–36 nt) was obviously higher in the Post-selection repertoires compared with that in the Pre-selection repertoires in all the four T cell subsets. (F) There were no differences in the degree of enrichment in short TCRβ CDR3s among the four T cell subsets. (G) TCRβ CDR3s clonotypes with reduced insertions were enriched during thymic selection in all the four T cell subsets. Data were presented as the mean ± SD values, and compared using the unpaired t-test. ***P < 0.001(two-tailed).