Table 1.

Primer composition and annealing temperatures for the PCR amplifications.

Primer position	Composition of primer pairs	Annealing temperature
V1-V3 Outer	F_AGRGTTYGATYMTGGCTCAG _ATTACCGCGGCTGCTGGC	61°C
V1-V3 Inner	F_GCATGCCTAACACATGCAA R_GCTGCTGGCACGGAGTTAG	63°C
V3-V5 Outer	F_CCTACGGGAGGCAGCAG R_CCGTCAATTCMTTTRAGT	58°C
V3-V5 Inner	F_AAGGCCTTCGGGTTGTAA R_CCTTTGAGTTTCAGCCTTGC	62°C