Figure 4.

Costimulatory blockade of B7/CD28, but not CD40/CD154, reduces numbers of transferred CD8⁺CD122⁺PD-1⁺ Tregs and frequency of IL-10-expressing Tregs in recipient mice. C57BL/6 mice received CD8⁺CD122⁺PD-1⁺ Tregs derived from Thy1.1⁺ mice (C57BL/6 background). They were then transplanted with BALB/c skin and treated with either MR1 or CTLA4-Ig. Two weeks later, transferred CD8⁺Thy1.1⁺ Tregs were quantified via FACS analysis (A) or stained for intracellular expression of IL-10. Histograms showing percentages of IL-10⁺ cells were gated on transferred CD8⁺Thy1.1⁺ population with one representing set of FACS data (B). Data are presented as mean ± SD from one representative of three separate experiments (C). *P < 0.05.