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. 2019 Feb 26;10:294. doi: 10.3389/fimmu.2019.00294

Figure 2.

Figure 2

In vivo uptake of Xcl1-(OVA SLP)-Fc in skin draining LN. WT and Batf3 KO mice were injected intradermally in the footpad with a mix of 50 μg of CpG and 6 μg of Alexa 488-labeled Xcl1-(OVA SLP)-Fc or Xcl1-Fc fusion proteins. Inguinal LNs were harvested 16 h post injection and the uptake of labeled fusion proteins was measured in different populations of APCs, (A) gating strategy for identifying CD103+ and CD8α subtypes in CD11c+B220negDCs isolated from inguinal LNs. (B) Uptake of labeled Xcl1-fusion proteins by CD8α DCs (left), CD103+ DCs (right), and (C) B220+ B cells (left), and CD11b macrophages (right). Data are shown as mean +/- SEM (n = 3–4 mice/group). Results are representative of two independent experiments. ***p < 0.001, ****p < 0.0001.