Figure 4.

Ex vivo function of TILs following Xcl1-fusion proteins vaccination. (A) Frequencies of OVA-specific CD8⁺ T cells in the spleens harvested 10 days post vaccination of mice challenged with B16.OVA on day 0, adoptively transferred with OT-I cells on day 7 and vaccinated on day 8. Data is shown as mean ± SEM (n = 5 mice/group). Results are representative of two independent experiments. (B) Frequencies of H-2Kb/OVA tetramer positive CD8⁺ T cells present in the B16.OVA tumors of vaccinated mice (left panel), and absolute number of SIINFEKL-specific CD8⁺ T cells per 5 mm³ of tumor mass (right panel). Data is shown as mean ± SEM (n = 5 mice/group). Results are representative of two independent experiments. (C) Representative plots depicting the IFNγ content of CD3⁺CD8⁺ T cells isolated from B16.OVA tumors 10 days post-vaccination and restimulated with or without SIINFEKL peptide. (D) Frequencies of IFNγ⁺ CD8⁺ T cells and (E) of CD107a⁺ CD8⁺ T cells isolated from B16-OVA tumors. Data is shown as mean ± SEM (n = 5 mice/group). Results are representative of two independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.