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. 2019 Jan 9;60(3):636–647. doi: 10.1194/jlr.M091736

Fig. 3.

Fig. 3.

CYP-like proteins are responsible for PUFA metabolite production in Leishmania. A: mRNA expression of CYP1, CYP2, and CYP3 genes in procyclic and metacyclic promastigotes determined by RT-qPCR. Data shown are from five experiments conducted in triplicate; means ± SEMs are shown (n = 5). B: Concentration of AA-derived metabolites in CHO cells expressing recombinant CYP1, CYP2, or CYP3 determined after cell media supplementation with AA. Data shown are from six experiments conducted in duplicate; means ± SEMs are shown (n = 6). *P < 0.05 and **P < 0.01 significantly different from the empty group. C: Concentration of DHA-derived metabolites in CHO cells expressing recombinant CYP1, CYP2, or CYP3 determined after cell media supplementation with DHA. Data shown are from six experiments conducted in duplicate; means ± SEMs are shown (n = 6). *P < 0.05 significantly different from the empty group.