Fig. 4.
Lipids extracted from Leishmania promote the synthesis of lipid mediators by BMDMs. A: Production of PUFA metabolites assessed by LC/MS/MS in BMDMs treated with 0% (M0), 50%, or 100% of lipids extracted from 1 × 108 procyclic and metacyclic promastigotes. Data shown correspond to three experiments conducted in triplicate; means ± SEMs are shown (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 significantly different from the M0 group. B: Production of proresolving mediators assessed by LC/MS/MS in BMDMs treated with 0% (M0), 50%, or 100% of lipids extracted from 1 × 108 procyclic and metacyclic promastigotes. Data shown are from three experiments conducted in triplicate; means ± SEMs are shown (n = 3). **P < 0.05 and ***P < 0.01 significantly different from the M0 group and ###P < 0.001 significantly different from the lipid extracts of the procyclic group.