Fig. 8.

ASM deficiency impacted the early reactive astrogliosis after brain trauma. Brain tissue samples were prepared from the injured brain cortex of WT, ASM KO, and WT mice treated with Reclast (2 mg/kg, ip). Sham-injured animal brain (sham) was used as a control. A: Equal amount of sample (30 μg) was loaded into the lane. The expression of panastrocyte marker Aldh1l1 and reactive astrocyte marker GFAP were determined by Western blotting. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Representative data are from six independent experiments. B: Quantification of the normalized GFAP protein expression using ImageJ software. Data are means ± SE. * P < 0.05 (n = 6 compared with sham); ^# P < 0.05 (n = 6, WT versus ASM KO).