Fig. 1.

Mogat1 gene expression and MGAT activity are increased during fasting-induced steatosis and are PPARα dependent. Male C57BL/6J mice were fasted for the times indicated or given free access to food (Fed) prior to euthanization. A: Liver triglycerides are increased during prolonged fasting in mice compared with fed controls. B: Gene expression of Mogat1, but not Mogat2, is significantly upregulated during fasting. C: MGAT activity was measured in hepatic membranes from fasting or fed mice and total activity was significantly increased after 18 and 24 h of fasting. D: Mogat1 induction during fasting (24 h) is PPARα dependent. Data are shown as mean ± SE; *P < 0.05 from fed state unless otherwise indicated; n = 3–6.