Fig. 3.

Diazirine alkyne probes regulate cholesterol homeostasis. U2OS-SRA cells (A, B) or CYP27A1-deficient human skin fibroblasts (C, D) were treated for 14 h in starvation media containing 10 μM probe or cholesterol in ethanol. HMGCS (A, C) and ABCA1 (B, D) mRNA expression was determined by qPCR relative to Rplp0. The LXR agonist T0901317 (T-cmpd) is shown as a positive control. Values are normalized to vehicle. Bars: mean + SEM, n = 5 (A, B) or n = 3 (C, D). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05 for comparisons indicated using one-way ANOVA followed by Dunnett’s test.