Whole-mount survey of β-Gal-stained adult SPL reporter mouse organs. A: Whole brain. B: Olfactory bulb, showing staining of specific olfactory ganglia. C: Forebrain. D: Midbrain. E: Cerebellum and brainstem. F: Hindbrain. G: Basicranium, showing positively stained trigeminal nerve ganglia and unstained pituitary. H: Spinal cord, showing gray matter staining. I: Thymus and heart. Thymus is intensely stained. The pin is within the ventricular wall of the bisected heart, and the view shows the interior of the ventricle. J: Kidney, showing strong staining in the renal pelvis. K: Bladder, showing epithelial staining. L: Female gonads, including ovaries, fallopian tubes, and uterus. M: Foot. N: Skin. O: Preputial gland. P: Ribcage. Q: Liver. R: Stomach. S: Jejunum, with a Peyer’s patch in the center and showing less staining than the surrounding jejunal tissue. T: Ileum. U: Brown adipose tissue. V: Harderian gland and eye. W: Adrenal gland. X: Tail. Data are representative of results obtained from four animals (two male and two female). Reproduced with permission from ref. 15.