Table 1.
P450 | Kinetic parameters for DEF |
|||
---|---|---|---|---|
Single plasmid expression |
Dual plasmid expression |
|||
KM (μM) | Vmax (RFU/sec) | KM (μM) | Vmax (RFU/sec) | |
CYP6M2 | 0.4 ± 0.02 | 902.2 ± 106.99 | 0.5 ± 0.01 | 292.5 ± 38.05 |
CYP6P3 | 0.7 ± 0.10 | 54.4 ± 6.52 | 0.9 ± 0.09 | 14.8 ± 4.89 |
CYP6P2 | nt | nt | 1.4 ± 0.02 | 300.6 ± 12.00 |
CYP6P4 | nt | nt | 1.0 ± 0.04 | 24.2 ± 1.26 |
CYP9J5 | nt | nt | 3.4 ± 0.21 | 265.1 ± 28.75 |
CYP6Z2 | nt | nt | 0.5 ± 0.3 | 43.7 ± 8.9 |
nt = not tested; (mean ± SD).