Figure 1.

Metformin reduces cell proliferation in SH-SY5Y cells. (a) Cells were treated with various concentrations of metformin (0.5, 1, 5, 10, and 20 mM) in serum starvation culture condition for 24 h. (b) Cells were treated with 5 mM metformin in serum starvation culture condition at different times (3, 6, 12, and 24 h). Cell proliferation was determined using MTT assay. Data represented the means ± S.E.M. of three independent experiments. ^∗p<0.05, ^∗∗p<0.01 versus control untreated group.