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. 2019 Feb 19;2019:1765182. doi: 10.1155/2019/1765182

Figure 5.

Figure 5

Metformin induces neuronal differentiation through cdk5/sox6 crosstalk. (a) Cells were incubated with or without 5 mM metformin for 0, 12, and 24 h in serum starvation culture condition. Protein expression of cdk5 and sox6 was determined using western blot analysis. (b) Quantitative results of Cdk5 and Sox6 expression in cells treated with or without 5 mM metformin were analyzed by Image J software. (c) Cdk5 and sox6 expression levels of cells treated with or without 5 mM metformin and 10 μM roscovitine for 24 h. (d) Quantitative results of Cdk5 and Sox6 expression in cells treated with or without 5 mM metformin and 10 μM roscovitine were analyzed by Image J software. (e) Cdk5 expression levels of cells treated with (+) or without (-) 5 mM metformin, in the present (+) or absent (-) of 10 mM NAC. In all western blot analysis, β-actin was used as endogenous control. Data represented the means ± S.E.M. of three independent experiments. ∗p<0.05, ∗∗p<0.01 versus control untreated group. Met: metformin, Rosc: roscovitine.